Subcellular Localisation, Protein Interactions, and RNA Binding of Potato mop-top virus Triple Gene Block Proteins
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چکیده
منابع مشابه
Production of Polyclonal Antibodies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein 1
The gene encoding the triple gene block protein 1 (TGBp1) of Potato mop-top virus (PMTV) was cloned into expression vector pQE32 tagging the protein with 6xHis on the N-terminus. When the gene was enshortened on its 3¢-end by two different restriction digestions, efficient and high yield bacterial expression was achieved in both cases, as shown by sodium dodecyl sulphate polyacrylamide gel elec...
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Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. In this study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of Potato mop-top virus (PMTV), was detected for the first time in PMTV-infected plants and found to be tyr...
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The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...
متن کاملCell-to-Cell Movement and Protein Interactions of Potato mop top virus
27 28 Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases 29 in plants but little is known about involvement of tyrosine kinases in plant virus infection. In this 30 study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of 31 Potato mop-top virus (PMTV), was detected for the first time in PMTV-infected plants and 32 fo...
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Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified ...
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ژورنال
عنوان ژورنال: Virology
سال: 2002
ISSN: 0042-6822
DOI: 10.1006/viro.2002.1435